A targeted sequence panel for detecting 34 vertebrate virus families at once

The challenge in detecting a set of viruses simultaneously in clinical samples lies in the large variety of their genomes, i.e. there is no gene that is present in all viruses. With the advent of new sequencing technologies, including high throughput parallel sequence analysis, it became possible to process more sequence data. So much more…Continue reading A targeted sequence panel for detecting 34 vertebrate virus families at once

MIME: Mutational Interference Mapping Experiment – RNA viruses

MIME (Mutational Interference Mapping Experiment) is used to investigate the interaction of RNA with its respective interaction partners in greater detail. In this approach, the RNA of interest is randomly mutated and amplified. The vast pool of resulting mutated RNAs is then mixed with a possible interaction partner, e.g.  a protein, is added. Next, the…Continue reading MIME: Mutational Interference Mapping Experiment – RNA viruses